Influence of Pioglitazone on the Expression of Adipor on Aortic in Hyperlipidemia Rats

Objective To observe the expression of AdipoR on the aorta in hyperlipidemia rats,and to investigate the influence of pioglitazone on the expression of AdipoR on aortic in hyperlipidemia rats,and the possible mechanism of anti-atherosclerosis.Methods 26 male SD rats were randomly divided into the high-fat diet group(17)and control group(9).high-fat diet group was fed with hypercholesterol diet for 12 weeks,then the serum lipid level was measured in the all rats,and to know whether the model of rats with hyperlipidemia was constructed by feeding hypercholesterol diet,then 17 male SD rats were randomly devided into two groups:model group,high hyperlipidemia with pioglitazone group.By intragastric administration,pioglitazone group rats were given pioglitazone(0.6mg/ml)for four weeks,then the serum lipid levels of all rats were measured and the aorta was taken for pathologic analysis.Serum adiponectin was detected by ELISA.AdipoR was measured by Western Bolt and reverse transcription polymerise chain reaction(RT-PCR).Results After 12 weeks of feeding,compared with control group,the high-fat diet group show significantly higher levels of total cholesterol(TC),triglyceride(TG)and low-density lipoprotein cholesterol(LDL-C)(P0.01).After 4 weeks' treatment,as compared with the model group,the level of TC,TG in the pioglitazone group was obviously lower(P 0.01),the level serum of adiponectin was significantly increased(P0.05),and the mRNA expression of both AdipoR1(P0.05)and AdipoR2(P0.01)were significantly increased,and the protein expression of both AdipoR1 and AdipoR2 were significantly increased(P0.05).Conclusion Dcreased adiponectin and its receptors may be involved in vascular impairment in rats with high-fat diet induced hyperlipidemia.Pioglitazone has anti-atherosclerotic effect,the effect may be related to increased serum adiponectin and vascular expression of adiponectin receptor.