Biologically Active Chicken Interleukine-18 Expression in Insect Cell/Baculovirus Expression System

cDNA fragment encoding the mature chIL-18 was subcloned into transfer plasmid pMelBac B.The purified positive plasmid rpMelBacB ChIL-18 was co-transfected to sf9 insect cells with lineal baculovirus gene(Bac-N-Blue TM DNA)by liposome.After screening by blue plaque for four times,the purified recombinant baculovirus named rBaculovirus ChIL-18 was obtained.Sf9 cells were infected with the rBaculovirus ChIL-18 and incubated at 28℃.The cells were harvested at 24,48,72,96,120 and 144 hours post-inoculation respectively.The lysates of the cells were analyzed by SDS-PAGE and western blot.It was demonstrated that chIL-18 mature protein was successfully expressed in the eukaryotic expression system and the recombinant protein had a molecular weight approximately 23kDa.;