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Study on hTERT expression of hepatocarcinoma cells inhibited by RNA interference in vitro

Huang Wen-fang

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Abstract

Objective To investigate the hepatocarcinoma therapy by RNA interference, we used siRNA to inhibit hTERT gene. expression in vitro.Methods We constructed a small interference RNA (siRNA) homologous to hTERT gene (siRNA-hTERT), transfected it into hepatocarcinoma cell 7721 to 1induce RNAi, then detected hTERT gene expression in both siRNA-hTERT treatment groups and control groups.Results The expression of hTERT was obviously inhibited by RNAi. The inhibition rate of cell growth was 37.5%. The phase of cell cycle indicated that the S phase decreased, while G1/G0 phase increased. The mRNA level of hTERT was decreased from 99.4% to 53.1%. Its protein expression reduced from 86.3% to 46.6%.Conclusion siRNA-hTERT effectively inhibits hTERT gene expression and cell growth.

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What this paper is about

Objective To investigate the hepatocarcinoma therapy by RNA interference, we used siRNA to inhibit hTERT gene. expression in vitro.Methods We constructed a small interference RNA (siRNA) homologous to hTERT gene (siRNA-hTERT), transfected it into hepatocarcinoma cell 7721 to 1induce RNAi, then detected hTERT gene expression in both siRNA-hTERT treatment groups and control groups.Results The expression of hTERT was obviously inhibited by RNAi. The inhibition rate of cell growth was 37.5%. The phase of cell cycle indicated that the S phase decreased, while G1/G0 phase increased. The mRNA level of hTERT was decreased from 99.4% to 53.1%. Its protein expression reduced from 86.3% to 46.6%.Conclusion siRNA-hTERT effectively inhibits hTERT gene expression and cell growth.

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Available abstract

Objective To investigate the hepatocarcinoma therapy by RNA interference, we used siRNA to inhibit hTERT gene. expression in vitro.Methods We constructed a small interference RNA (siRNA) homologous to hTERT gene (siRNA-hTERT), transfected it into hepatocarcinoma cell 7721 to 1induce RNAi, then detected hTERT gene expression in both siRNA-hTERT treatment groups and control groups.Results The expression of hTERT was obviously inhibited by RNAi. The inhibition rate of cell growth was 37.5%. The phase of cell cycle indicated that the S phase decreased, while G1/G0 phase increased. The mRNA level of hTERT was decreased from 99.4% to 53.1%. Its protein expression reduced from 86.3% to 46.6%.Conclusion siRNA-hTERT effectively inhibits hTERT gene expression and cell growth.

Key concepts: RNA interference, Telomerase reverse transcriptase, Small interfering RNA, Transfection, Molecular biology, Gene expression, In vitro, RNA

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