Comparative study of the growth and enzyme production of Rhizopus mycelium in two different distriller's yeast

The impact of environment on the growth and enzyme production of Rhizopus mycelium was studied through the comparative studies of Anqi distriller's yeast and Huizhou Distriller's yeast,by using two different culture methods,solid plate method and liquor shaking method.The results showed the difference of the two distriller's yeasts was not obvious in the colonial morphology characteristics of Rhizopus,but the Rhizopus growth speed of Anqi distriller's yeast was slightly higher.The suitable growing temperature was 20～25℃ for Huizhou and Anqi distriller's yeast,and the optimal pH value was both 4.When alcohol concentration(AC) of culture medium reached 6%,the mycelium growth of both was restrained apparently.Rhizopus spore would stop spouting when AC reached 8%.The optimal shaking speed for the growth of Rhizopus mycelium was 100～150r/min.In general,Rhizopus mycelium growth in Anqi distriller's yeast was better than that in Huizhou distriller's yeast.