Differences of rat adipose derived stromal cells osteogenesis in different induction time

Objective To observe the effect of induction time selection on the differentiation of rat adipose derived stromal cells(ADSCs) into osteoblasts.Methods The induced group was added into conditioned medium containing 1×10-8mol/L dexamethasone,10mmol/L beta-sodium glycerophosphate and 50mg/L ascorbic acid at the same time of seeding,at 50%-60% confluence,at 85%-90% confluence,respectively.The A,B,C group of non-induction group was cultured with ordinary medium.Results After calcium-cobalt staining,in induced group a majority of cells were positively stained,and the quantity of positive cells reached the peak at 85%-95% confluence.Non-induced group was no positive cells;Alizarin red stain were visible red nodules,The induction group formed nodules earlier at cells of 85%-95% confluence;Intracellular alkaline phosphatase activity detection showed that intracellular alkaline phosphatase activity markedly increased after inducing by osteogenic inducer than non-induced group.Conclusion After induced ADSCs can differentiate into osteoblasts;Induced alkaline phosphatase activity within cells was significantly higher than non-induced group;Under the same initial seeding density,the higher the ADSCs contact with each other,the stronger the osteogenesis is.