Effect of highly effective selection of mouse CD40 small interference RNA and on the expression of CD40 in mouse lymphocytes

Objective: To select highly effective mouse CD40 small interfering RNA(siRNA)by the technology of interfering RNA.Methods: Three specific chemically synthesized siRNAs targeting on CD40 of mouse were designed and were transfected into lymphocytes with Lipofectamine RNAiMAX via reverse transfection,while nonspecific siRNA and untreated cell served as the control separately,The expression of CD40 and CD40 mRNA in lymphocytes were measured by flow cytometry and real-time fluorogenic quantitative PCR(RFQ-PCR) respectively after 24 h of transfection.The expression of CD40 protein levels were assessed by Western blot after 48 h of transfection.Results: Compared with the experimental and controls,siRNA group showed significantly down-regulation of CD40 mRNA and protein in lymphocytes.The total inhibitory rate of CD40 expression for siCD40-1,siCD40-2,siCD40-3 were 69%,78% and 41% respectively.There was no significant difference found in controls.Conclusion: siRNA of CD40 could specifically down-regulate the expression of CD40 mRNA and protein in mouse lymphocytes.siRNA-2 has the highly effective inhibition.