The Changes of Expression of mGluR5 Induced by Acute Cerebral Ischemia in Rats

Objecive: To observe the expression of mGluR5 and the dynamic regulation in the cortex of rats with focal cerebral ischemia in different phases and to investigate its significance in acute ischemia.Methods:Thirty-five male Wistar rats were raodomly divided into 11 groups: The normal contral,sham operation and ischemic operation(subdivided into 1,3,6,12 and 24-hour) by middle cerebral artery occlusion model.Brain specimens of the rats from each group were obtained for HE staining and immunohistochemistry staining.To observe the damage of neurons with light microscope and to judge the positive expression of neurons,and then to detect the changes of gray scale value in each group.Results:Compared with the normal and each sham control group,the expression of mGluR5 in each ischemia operation group was elevated 1h after ischemia and reached the peak within 12 hours after ischemia.However,there was was no significant difference between normal and sham control groups.Conclusion:Acute cerebral ischemia can cause up-regulated expression of mGluRs,suggesting that up-regulated expression of mGluR5 might be related with focal cerebral ischemia and it contributed to the development of cerebrovascular disease.According to the results of previous study,however,we presume the up-regulated expression might be the responsive increase in order to remove glutamate,which is a protect mechanism for maintain balance of organism.