Changes of hepatic pathology and AFP level during hepatic cirrhosis and hepatocarcinogenesis in rat

Objective To investigate the significance of the change of hepatic pathology and AFP level during hepatic cirrhosis and hepatocarcinogenesis in rat.Methods Wistar male rats were respectively selected to establish the model of hapatic carcinoma and cirrhosis with the reagents of DENA,carbon tetrachloride and olive oil.The tissues of liver and spleen as well as peripheral vein blood were respectively obtained before induction,2 weeks,4 weeks,8 weeks,14 weeks,18 weeks and 21 weeks after induction.Histopathology of liver and spleen was observed with HE staining,serous AFP level was detected with ELISA method.Results Hepatic pseudolobule and congestive splenomegaly occurred at 18 weeks during hepatic cirrhosis induction,pathological karyokinesis,huge karyosomes,hepatic cancerous tuberosis and splenic congestion occurred at 14 weeks during hepatic carcinoma induction.The AFP level increased at 4 weeks during hepatic cirrhosis induction,there was significant difference between 4 week and 14 weeks(P 0.05).The AFP level increased at 2 weeks during hepatic cirrhosis induction,there was significant difference between 2 weeks and 8 weeks(P 0.05).The AFP level in rat with hepatic carcinoma was significantly higher than that of rat with hepatic cirrhosis at 8 week(P 0.05).Conclusion The emergence time of pathological change and damage degree in liver are different from various inducements,liver damage from DENA is more severe than that of carbon tetrachloride.Serous AFP level is dynam ically measured when liver cells are damag eous to virus,bacteria,toxins and chemical substances etc;it is reduplicative biological significance to evaluate the damage degree and canceration of liver cells.