ScholarLens Back to search
2007•Progress in Veterinary MedicineRequires access

Cloning and Sequence Analysis of IFN-γ Gene Gene in Guangxi Water Buffalo

Qi Liu

Open publisher page 0 citations

Abstract

Total RNA was isolated from cultured water buffalo peripheral blood lymphocytes stimulated with ConA.The cDNA of water buffalo interferon-γ(IFN-γ) cDNA was amplified by RT-PCR,and then cloned into pMD18-T and sequenced.The ORF of the cloned water buffalo IFN-γ gene was 501bp in length,encoding a mature polypeptide with 166 amino acids.Compared with other IFN-γ,the nucleotide homologies between the water buffalo IFN-γ gene and the cattle IFN-γ genes were higher than 97%,the amino acid homologies were higher than 96%.The homologies with other animals were step down in accordance with its genetic relationship.The results showed that the cloning plasmid was successfully constructed,and it is the basis for further study and application of Guangxi water buffalo IFN-γ.

About this research paper

What this paper is about

Total RNA was isolated from cultured water buffalo peripheral blood lymphocytes stimulated with ConA.The cDNA of water buffalo interferon-γ(IFN-γ) cDNA was amplified by RT-PCR,and then cloned into pMD18-T and sequenced.The ORF of the cloned water buffalo IFN-γ gene was 501bp in length,encoding a mature polypeptide with 166 amino acids.Compared with other IFN-γ,the nucleotide homologies between the water buffalo IFN-γ gene and the cattle IFN-γ genes were higher than 97%,the amino acid homologies were higher than 96%.The homologies with other animals were step down in accordance with its genetic relationship.The results showed that the cloning plasmid was successfully constructed,and it is the basis for further study and application of Guangxi water buffalo IFN-γ.

Why it matters

A significance statement is not available in the OpenAlex record.

Key contribution

A contribution statement is not available in the OpenAlex record.

Method / approach

Method details are not available in the OpenAlex metadata.

Main findings

Findings are not separately available in the OpenAlex metadata.

Limitations

Limitations are not available in the OpenAlex metadata.

Applications

Application details are not available in the OpenAlex metadata.

Available abstract

Total RNA was isolated from cultured water buffalo peripheral blood lymphocytes stimulated with ConA.The cDNA of water buffalo interferon-γ(IFN-γ) cDNA was amplified by RT-PCR,and then cloned into pMD18-T and sequenced.The ORF of the cloned water buffalo IFN-γ gene was 501bp in length,encoding a mature polypeptide with 166 amino acids.Compared with other IFN-γ,the nucleotide homologies between the water buffalo IFN-γ gene and the cattle IFN-γ genes were higher than 97%,the amino acid homologies were higher than 96%.The homologies with other animals were step down in accordance with its genetic relationship.The results showed that the cloning plasmid was successfully constructed,and it is the basis for further study and application of Guangxi water buffalo IFN-γ.

Key concepts: Cloning (programming), Gene, Complementary DNA, Biology, Water buffalo, Molecular biology, Plasmid, Nucleic acid sequence

Related papers

Related paper 1Related paper 2Related paper 3
Back to paper searchBrowse research topicsOriginal source
Cloning and Sequence Analysis of IFN-γ Gene Gene in Guangxi Water Buffalo — Research Paper | ScholarLens