The Mechanism Study on Acupuncture Affecting on Cardiomyocyte Apoptosis in the Type 2 Diabetic Rats

Objective:To study affects of acupuncture on cardiomyocyte apoptosis in the model rats of type 2.Methods:The model of type 2 diabetic rats was made and divided into three groups at random:the diabetic rats without treatment,the diabetic rats with acupuncture-treatment and the normal rats.40 days later,the blood samples were collected to exam.With the help of microscope,we observed how cardiomyocyte apoptosis occurred and examed expression of inflammatory factors,free fatty acid(FFA) and nitric oxide(NO) in the blood.Results:(1)The fasting plasma glucose(FPG),fasting plasma insulin(FINS) and HomaIR for the groups of diabetic rats without treatment,the diabetic rats with acupuncture-treatment and the normals have been down,while HBCI has raised(P0.01,P0.05)in turn.(2)Although plasma triglycerides(TG),the total cholesterol(TC),the LOW-density lipoprotein cholesterol(LDL-C),FFA,NO,TNF-α,IL-6,CRP,PAI-1 of the diabetic rats with acupuncture-treatment were obviously lower than that of the diabetic rats without treatment,these datas had no differences between the diabetic rats with acupuncture-treatment and the normal contrals.The high-density lipoprotein cholesterol(HDL-C) of the diabetic rats with acupuncture-treatment is obviously higher than that of the diabetic rats without treatment(P0.01).(3)The expression of cardiomyocyte apoptosis of the diabetic rats without treatment were much more higher than the diabetic rats with acupuncture-treated and the normal controls(P0.01).But the datas of the diabetic rats with acupuncture-treatment had no statistical difference in comparision to the normal controls(P0.05)(5)With the help of microscope,it could be observed that myocardial cells arranged disorde and decreased,karyopyknosis in diabetic rats,while Structural change of the diabetic rats acupuncture-treatment were lighter in comparion to the diabetic rats without treatment.Conclusion:(1)The acupuncture may improve diabetes cardiomyocyte apoptosis obviously,its mechanism is possibly associated with the decreacing of inflammation and the nitric oxide,the free fatty acids,the plasmaglucose.