Flavonoid content of the leaves and pollen of male Ginkgo biloba plants.

【Objective】 To provide a theoretical evidence for the development and utilization of Ginkgo biloba male plant germplasm resources,the major flavonoid components and content of the leaves and pollen of 46 male ginkgo plants from Jiangsu province were studied. 【Method】 The flavonoid aglycone content of the methanol (ethanol)-hydrochloric acid solution of leaves and pollen of different male strains were determined by HPLC. The flavonoid glycoside content of the samples were calculated according to the three-factor method. 【Result】 Significant differences were existed between the total flavonoid content of leaves and pollen in different male plants. Four strains from Yangzhou (Yangzhou 03,04,10 and 20),two strains from Taizhou (Taizhou 08 and 13) and two strains from Xuzhou (Xuzhou 04 and 09) met with the standard of leaf utilization,and ten strains from Yangzhou (Yangzhou 02,03,04,08,09,10,12,15,18 and 20) met with the standard of pollen utilization by setting the choice threshold of flavonoid aglycone and total flavonoids content. The leaves and pollen of different male ginkgo plants both contained three flavonoid aglycones (quercetin,kaempferol and isorhamnetin),but the amount of each differed between leaf and pollen. There was little difference in the content of individual flavonoid aglycones in leaves,whereas kaempferol was the main flavonoid aglycone in pollen. The average total flavonoid content of pollen was about 1.42 times that of the leaves. 【Conclusion】 According to the choice of setting targets,either the leaf or pollen of male ginkgo plants were selected for production based on the analysis of flavonoid aglycone and total flavonoid contents. The results have provided a theoretical basis for the use of male ginkgo plant resources.