The effect of Naloxone on apoptosis of neuron after the cerebral ischemic-reperfusion injury in rats

Objective:The aim of this study was to observe the protective effect of Naloxone on the neural apoptosis after global cerebral ischemic-reperfusion injury in rats.Methods:Thirty-six male Wistar rats were used in this study..All rats were divided into three groups at random.Four-vessel occlusion was used to establish the model of global cerebral ischemia reperfusion in the study groups and control group.The Naloxone was injected into abdomen on the different time-spot in treated groups.On the 48h time-spot after reperfusion hippocampus was separated for the detection of apoptosis and Bcl-2,Bax protein by the Flow Cytometry.Results:There was significant difference among the treatment groups and the control group(p0.01)for the positive cell number.There was significant difference among the treatment groups and the control group(p0.01)for the Bcl-2 and Bax detection.Conclusion:The Naloxone may reduce the numbers of apoptotic nervous cell after global cerebral ischemic-reperfusion injury in rats.