Effect and Mechanism of IFN-α1b on Proliferation of Endometrial Adenocarcinoma Cell Line JEC

Objective To study the inhibitory effect of IFN-α1b on Endometrial adenocarcinoma cell line JEC and its mechanism by measuring the change of cell cycle and detecting the expression of p21, cyclin A and cyclin E.Methods After treated by IFN-α1b at different concentrations 24 hours, the amount of cell was measured by cell count to learn the effect of IFN-α1b on the proliferation of JEC cell; cell cycle phase and expression of p21, cyclin A and cyclin E were analyzed by flow cytometry(FCM). Results IFN-α1b all have inhibitory effects at different concertrations,the strongest of which is 500U/ml. FCM shows that cells are accumulated in S phase in cell cycle;the expression of p21 and cyclin E increase but cyclin A reduces.Conclusion IFN-α1b can inhibit proliferation of the JEC cell and its mechanism relates to cell arrest in S phase which is probably caused by the increased expression of p21 and the reduced expression of cyclin A.