A Clinical Pathological Analysis For 22 cases of Diffuse Large B-cell Lymphona(DLBCL)

Objective To observe the morphological and immunohistochemical featuees of Diffuse Large B-cell Lymphoma(DLBCL) Materials and Methods:Twenty-two cases of DLBCL,which were diagnosed by the criterion of new WHO classification,were selected in Pathological Department.The tissues of all cases were fixed in buffered formalin and embedded in paraffin wax.Morphologic observation for HE,PAS,and immunohistochemical analyses for CD3、CD5、CD20*******,CD30、cyclinD1 and Iysozyme antigens were performed.Results;Every variant of DLBCL has it's own histological characteristics.Immunostaning showed that the tumor cells in all cases of DLBCL were positive for CD20,and were negative for.in which.3 caese of ALDL expressed CD30 Morphologically,tumor cells showed the nodular,diffuse,or sheet pattern of proliferation.Conclusions:The diagnosis and differential diagnosis for Diffuse Large B-Cell Iymphoma( DLBCL) have been a difficult problem for pathologist in a long time.According to time criterion of new WHO classification,the synthetic diagnostic scheme,which comprises standary morphologic observation,systematic IBC analyses,can improve the confirmed diagnosis rate of DLBCL dramatically.All of these ate helpful to the therapy and prognosis for DLBCL,even foe other categories.