2002•Europe PMC (PubMed Central)Requires access
Fabrication of cDNA Microarray for the Gene Expression in Leukemia K562 Cell Line
Ji Zhu, Wenli Ma, Yanbin Song, Qinghua Wu, Yao Ruhua, Wenling Zheng
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Abstract
3256 cDNA fragments were isolated from human leukemia K562 cells, induced with hydroxyurea by restriction display PCR (RD-PCR). DNA microarray of K562 cell gene expression profile was prepared. Influences of different spot solutions and treatments on DNA attachment and optimal DNA concentration were studied. The results showed that DNA attachment rate was 95% when DMSO was used as spot solution, DNA concentration was 0.3μg/μL, microarray was crosslinked by UV ray (150 mJ) and dried at 80 ℃ for 2 h. A good crosslinking effect was achieved using the DNA microarray of K562 cell gene expression profile prepared in this study.