Construction of Recombinant Lentivirus Vector Carrying hTERT Gene and Its Packaging and Expression

Objective To construct the recombinant lentivirus vector containing hTERT gene and detect the expression of hTERT in 293T cells.Methods The hTERT gene amplified by PCR was inserted into the lentivirus vector,the recombinant lentivirus vector was transfected into 293T cells with the packaging plasmids and the titre was determined.The expression of hTERT in 293T cells was determined by Western blot.Results The result proved that the recombinant lentivirus vector was constructed successfully and the titre of virus supernatant was 2.79×107 Tu/mL.Western blot showed the positive expression of hTERT in 293T cells which were infected by recombinant virus supernatant.Conclusion The recombinant lentivirus vector could be constructed and packaged successfully,the expression of hTERT gene was positive after the 293T cells were infected by recombinant virus supernatant.