The Effects of TNF-Alpha and Angiotensin II on Endothelial Cell Secreting PAI-1

Objective To investigate the effects of tumor necrosis factor Alpha (TNF-α) and angiotensin Ⅱ (Ang Ⅱ )on endothelial cell secreting tissue plasminogen activator(t-PA) and plasminogen activator inhibitor (PAI-1) .Methods Cultivated ECV304 cells were stimulated with TNF-a (5、 10、 25、 50、 100ng/ml) and Ang Ⅱ (5、 10、 25、50、 100nmol/L)for 24 hours. Tissue-type plasminogen activator (t-PA)and plasminogen activator inhibitor-1(PAI-1)in the supernatant of the cell culture were measured by enzyme linked immunosorbent assay (ELISA). Results The concentrations of t-PA had no statistically significant change, the ECV304 cells were stimulated by TNF-a or Ang Ⅱ . But PAI-1 was significandy increased by TNF-α (77.6±2.5ng/ml,versus 80.5 ±0.9±89.2±6.9±81.1 ± 1. 1±86.7 ± 5.6±100.9 ±11.0ng/ml), stimulated with TNF-α (5、 10、 25、 50、 100ng/ml). A distinct increase of PAI-1 was found (76. 8 ± 1.8ng/ml versus 79.2±0.9±80.1 ± 1.2ng/ml), stimulated with AngⅡ(50、10nmol/L). Conclusion TNF-α and Ang Ⅱ has no effects on t-PA released from ECV304. But, PAI-1 can be increased by TNF-α and Ang Ⅱ . TNF-α or Ang Ⅱ plays a role in the regulating of fibrinolysis.