The early protection of nimodipine on acute cerebral ischemia-reperfusion injury in rats

Objective To investigate the early protective effect of nimodipine on acute cerebral ischemia reperfusion injury.Methods Focal cerebral ischemia/reperfusion was induced by the middle cerebral artery occlusion(MCAO) method.30 male Wistar rats were randomly divided into three groups(Sham,Model,Nimodipine teatment group).The model group was induced by 2h ischemia following 2h reperfusion.The nimodipine treatment(5mg/kg) was given at the time of ischemia by intraperitoneal injection once an hour.At the end of the experiment,the abdominal aorta blood was obtained,and full brain tissue was saved.Infarct size by TTC staining of brain tissue slices was compared between groups.The activities of SOD,MDA and NO content were detected in serum and brain tissue.Results The infarct size of nimodipine treatment group was significantly decreased compared with model group.Serum biochemical indicators suggest that the SOD content of the model group was significantly lower than the sham group,and increased significantly in nimodipine treatment group.MDA and NO content was significantly higher in the Model group than that in the sham group.Nimodipine treatment significantly reduced the serum level of MDA and NO.Conclusion Nimodipine has a protective effect on acute cerebral ischemia and reperfusion injury,which is closely related to its anti-oxidative stress effect.