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Effect of varicocele on expressions of HIF-1α and Bax in germ cells of adolescent rats

Yue Wu

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Abstract

Objective To study the effect of varicocele(VC) on the expressions of hypoxia-inducible factor-1α(HIF-1α) and apoptosis-associated gene Bax in germ cells of adolescent rats,and investigate the mechanism of the infertility resulting from varicocele. Methods The varicocele model was established by partial ligation of the left renal vein in adolescent male Wistar rats. A total of 25 rats were randomly divided into 2 groups,namely varicocele group(n=15) and sham operation group (SOG,n=10). All the animals were killed 30 d after operation. The left testis was collected and the expression of HIF-1α and Bax was detected by immunohistochemical method. Results The expression of HIF-1α and Bax in left testis of varicocele group was significantly higher than that of sham operation group(P0.05) and positive correlation was found between them (r=0.479 2,P0.05). Conclusion Experimental varicocele can change the expression of factor-1α(HIF-1α) and apoptosis associated gene Bax in germ cells of adolescent rats.

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Objective To study the effect of varicocele(VC) on the expressions of hypoxia-inducible factor-1α(HIF-1α) and apoptosis-associated gene Bax in germ cells of adolescent rats,and investigate the mechanism of the infertility resulting from varicocele. Methods The varicocele model was established by partial ligation of the left renal vein in adolescent male Wistar rats. A total of 25 rats were randomly divided into 2 groups,namely varicocele group(n=15) and sham operation group (SOG,n=10). All the animals were killed 30 d after operation. The left testis was collected and the expression of HIF-1α and Bax was detected by immunohistochemical method. Results The expression of HIF-1α and Bax in left testis of varicocele group was significantly higher than that of sham operation group(P0.05) and positive correlation was found between them (r=0.479 2,P0.05). Conclusion Experimental varicocele can change the expression of factor-1α(HIF-1α) and apoptosis associated gene Bax in germ cells of adolescent rats.

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Available abstract

Objective To study the effect of varicocele(VC) on the expressions of hypoxia-inducible factor-1α(HIF-1α) and apoptosis-associated gene Bax in germ cells of adolescent rats,and investigate the mechanism of the infertility resulting from varicocele. Methods The varicocele model was established by partial ligation of the left renal vein in adolescent male Wistar rats. A total of 25 rats were randomly divided into 2 groups,namely varicocele group(n=15) and sham operation group (SOG,n=10). All the animals were killed 30 d after operation. The left testis was collected and the expression of HIF-1α and Bax was detected by immunohistochemical method. Results The expression of HIF-1α and Bax in left testis of varicocele group was significantly higher than that of sham operation group(P0.05) and positive correlation was found between them (r=0.479 2,P0.05). Conclusion Experimental varicocele can change the expression of factor-1α(HIF-1α) and apoptosis associated gene Bax in germ cells of adolescent rats.

Key concepts: Varicocele, Left renal vein, Immunohistochemistry, Apoptosis, Andrology, Ligation, Male infertility, Medicine

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