Effects of emodin on aquaporin 1 expression in acute lung injury in sepsis rats

Objective To investigate the effects of emodin on aquaporin 1(AQP-1)expression in acute lung injury in sepsis rats.Methods A total of 120 adult SD rats weighing 200-230 g were randomly divided into six groups,blank control group,sham group,model group,low dose emodin pretreatment group,middle dose emodin pretreatment group,high dose emodin pretreatment group,20 rats in each group.The rats with sepsis were induced by cecal ligation and puncture(CLP).The abdominal cavity in sham group was closed after cecal exploration operation.Each group was further divided into four time points:3hours,6hours,12 hours and 24 hours after the modeling,with 5rats in each time point,according to the specimen collection time.Rats were sacrificed at different time points to measure the expression of AQP-1mRNA by real-time fluorescence quantitative PCR and AQP-1protein by western-blot.Results AQP-1expression at 3hamong different groups showed no significant difference.After modeling,AQP-1at 6hdecreased gradually and reached the minimum 12 h.AQP-1of the middle and high dose emodin pretreatment groups at 12 hwere significantly higher than those of the same dose of other time points(P 0.01).There was no significant difference between the two groups(P0.05).Conclusion Sepsis caused the AQP-1expression of rats significantly lower.Early prophylactic use of middle or high dose emodin can significantly increase AQP-1expression.