The Vitro Study on Intervention of Glycyrrhizin and Dexamethasone in Podocytes Proliferation

Objective To establish podocyte injury model induced by puromycin aminonucleoside(PAN),observing the impact of glycyrrhizin(GL) and dexamethasone(DEX) on proliferation of normal podocyte and injured podocyte,and exploring whither GL was similar protective effect to DEX on podocyte injury in vitro.Methods Murine podocytes were divided into 6 groups:control group(apply RPMI-1640 at same quantity),PAN group(PAN 50mg /L),GL group(GL50,100,200,400,600,800mg /L),DEX group(DEX 1μmol /L),PAN + GL group(PAN 50mg /L + GL50,100,200,400,600,800mg /L),PAN + DEX group(PAN 50mg /L + DEX 1μmol /L).the proliferative podocyte cells were detected with MTT assayafter 24h or 48h treatment.Results Compared to control group,the proliferation of podocyte(A value) in PAN group was significantly lower 24h and 48h(P 0.01),the proliferation inhibition rate was 55.56% and 59.10% ;The A value in GL group was significantly lower 24h and 48h,except that the A value in GL50mg /L group was not decreased after 48h treatment compared to control group(P 0.05) ;the inhibition rate in lower dose of GL(100 ～ 400 mg /L) 48h was decreased comparable to 24h while it remain high level in higher dose of GL(600 ～ 800 mg /L) 48h.The A value in DEX group was significantly lower 24h and 48h(P 0.01),the inhibition rate was almost similar between 24h and 48h.Compared to PAN group,the A value in PAN + GL 50 ～ 600mg /L groups were significantly increased in a dose-dependent manner after 24h and 48h treatment(P 0.01),but the ability of podocyte proliferation was not strengthened with high dose(PAN + GL 800mg /L) group;The A value in PAN + DEX group was significantly enhanced both 24h and 48h(P 0.01).Conclusion PAN can inhibit podocyte proliferation severely,GL and DEX have slightly suppressive effects on podocyte proliferation,but both of them can improve proliferation of injured podocyte induced by PAN,while GL has same cytoprotective effect of DEX on injured podocyte cultured in vitro.