A SIMPLE MODEL OF SUBARACHNOID HEMORRHAGE IN RATS

Objective To build a simple animal model of subarachnoid hemorrhage. Methods Forty-two Wistar rats were randomly divided into three groups: SAH group, sham-operation group and control group. The rats in SAH group were produced by blood taken from the tail of rats then injected into cisterna magna, and those in the sham-operation group were injected with saline of the same volume, the normal control group without any injection. The assessment of the models were done by Lodffle’s neurobehavior score and the anatomy was observed as well. Results The neurobehavior scores in sham-operation group were higher than those of SAH group (t=4.78-10.30,P0.01). There was always blood in the basal cisterns with some spread over the hemisphere in rats of SAH group, it was not seen in control group and sham-operation group. Conclusion The establishment of SAH-rat models using autoblood is simple, reliable and repeatable.