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Genetic Toxicity Detection of Methanesulfonate(MMS) and Cyclophosphamide(CP) by Comet Assay with HepG2 and L5178Y Cells

Zheng­ping Liu

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Abstract

In order to explore the application value of HepG2 cells in the comet assay,the genetic toxicity of the direct mutagens methyl methanesulfonate(MMS) and indirect mutagens cyclophosphamide(CP) were evaluated respectively by comet assay with HepG2 or with L5178Y cells.The results show that comet assay with HepG2 cell can detect the genetic toxicity of MMS and CP in absence of exogenous S9activation system while the comet assay with L5178Y cells needs exogenous S9 activation system to detect the genotoxicity of CP.Therefore,the comet assay with HepG2 cell has a simpler procedure and wider application.

About this research paper

What this paper is about

In order to explore the application value of HepG2 cells in the comet assay,the genetic toxicity of the direct mutagens methyl methanesulfonate(MMS) and indirect mutagens cyclophosphamide(CP) were evaluated respectively by comet assay with HepG2 or with L5178Y cells.The results show that comet assay with HepG2 cell can detect the genetic toxicity of MMS and CP in absence of exogenous S9activation system while the comet assay with L5178Y cells needs exogenous S9 activation system to detect the genotoxicity of CP.Therefore,the comet assay with HepG2 cell has a simpler procedure and wider application.

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Available abstract

In order to explore the application value of HepG2 cells in the comet assay,the genetic toxicity of the direct mutagens methyl methanesulfonate(MMS) and indirect mutagens cyclophosphamide(CP) were evaluated respectively by comet assay with HepG2 or with L5178Y cells.The results show that comet assay with HepG2 cell can detect the genetic toxicity of MMS and CP in absence of exogenous S9activation system while the comet assay with L5178Y cells needs exogenous S9 activation system to detect the genotoxicity of CP.Therefore,the comet assay with HepG2 cell has a simpler procedure and wider application.

Key concepts: Comet assay, Methyl methanesulfonate, Genotoxicity, Comet, Ethyl methanesulfonate, Toxicity, Molecular biology, Chemistry

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Genetic Toxicity Detection of Methanesulfonate(MMS) and Cyclophosphamide(CP) by Comet Assay with HepG2 and L5178Y Cells — Research Paper | ScholarLens