An experimental study on the change of portal vein pressure in the process of establishing portal hypertension animal models in canines by partial portal vein ligation

Objective:To study a reasonable range on controlling portal vein pressure through the change of portal vein pressure in the process of establishing portal hypertension animal models in canines by partial portal vein ligation.Methods:Twenty four canines were divided three groups randomly,and every group had six cases.Ligations of the portal vein were performed in all canines.Intraoperatively the portal vein pressure was controlled in a range of 20.0～25.0 cmH2O in group A and 25.0～30.0 cmH2O in group B and 30.0～35.0 cmH2O in group C.The portal vein pressure was measured after 2 weeks,4 weeks,6 weeks,8 weeks,10 weeks,12 weeks and 14 weeks.The condition of esophageal varices gastric was observed after 14 weeks.Results:Portal vein pressure descended gradually in each group,and portal vein pressure was smooth after 14 weeks.In group A the portal vein pressure decreased from (22.87±1.43) cmH2O to (19.56±0.80) cmH2O (P0.05) after 14 weeks,and significant esophageal varices was not appeared in four canines,and no animals died;In group B the portal vein pressure decreased from (27.22±1.47) cmH2O to (21.81±0.99) cmH2O (P0.05) after 14 weeks,and significant esophageal varices was appeared in all canines,and one animal died;In group C the portal vein pressure decreased from (31.86±1.52) cmH2O decreased to (25.32±1.79) cmH2O (P0.05),and significant esophageal varices was appeared in all canines,and three animals died.Conclusions:In the process of establishing portal hypertension models in canines by partial portal vein ligation,the portal venous pressure should be controlled in a range of 25.0～30.0 cmH2O,in this range the esophageal varices of animal models was significant and death rate was lower.The portal hypertension animal models in canine can be used in a variety of experimental studies.