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2010•Heilongjiang xumu shouyiRequires access

Construct of the full-length DNA concatemer of CAV

Linlang Guo

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Abstract

To further study on the infectious DNA clone and biological characterization of CAV,a pair of primers was designed according to the full-length genomic sequence of Cux-1 strain of CAV based on GenBank data.The full-length genomic sequence of Cux-1 strain of CAV was amplified by PCR and cloned into pBluescript Ⅱ SK(+) vector.The full-length DNA clone of CAV were obtained,two full-length DNA of CAV were linked by incomplete enzyme cutting.The results showed that the two full-length DNA of CAV were straight-forward linkage by restriction identification and sequencing analysis.It illustrated that the full-length DNA concatemer of CAV were successfully received.

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What this paper is about

To further study on the infectious DNA clone and biological characterization of CAV,a pair of primers was designed according to the full-length genomic sequence of Cux-1 strain of CAV based on GenBank data.The full-length genomic sequence of Cux-1 strain of CAV was amplified by PCR and cloned into pBluescript Ⅱ SK(+) vector.The full-length DNA clone of CAV were obtained,two full-length DNA of CAV were linked by incomplete enzyme cutting.The results showed that the two full-length DNA of CAV were straight-forward linkage by restriction identification and sequencing analysis.It illustrated that the full-length DNA concatemer of CAV were successfully received.

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Available abstract

To further study on the infectious DNA clone and biological characterization of CAV,a pair of primers was designed according to the full-length genomic sequence of Cux-1 strain of CAV based on GenBank data.The full-length genomic sequence of Cux-1 strain of CAV was amplified by PCR and cloned into pBluescript Ⅱ SK(+) vector.The full-length DNA clone of CAV were obtained,two full-length DNA of CAV were linked by incomplete enzyme cutting.The results showed that the two full-length DNA of CAV were straight-forward linkage by restriction identification and sequencing analysis.It illustrated that the full-length DNA concatemer of CAV were successfully received.

Key concepts: Concatemer, Biology, Restriction enzyme, Genetics, DNA, Molecular biology, GenBank, clone (Java method)

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