Effect of Cinnamaldehyde on Proliferation and Apoptosis of a Heterologous Graft in Nude mice Bearing Human Gastric Carcinoma

Aim To investigate the cytotoxic effects of Cinnamaldehyde (CA) on cancer cell lines and effects on proliferation and apoptosis of heterologous graft in nude mice bearing human gastric carcinoma.Methods The antiproliferative effects of CA in seven human cancer cell lines were tested by MTT assay.The model of nude mice bearing developed xenografts of the SGC-7901 cell line were established and administered with different dose of CA and Carboplatin (CBP) by intraperitoneal injection (ip).The weights of the tumors and inhibitory rate of tumors of the mice were observed.The changes of SGC-7901 cell cycle of transplanted tumor and apoptosis rate were determined by flow cytometry (FCM).The morphological changes of apoptosis were observed by transillumination electron microscope (TEM).Results In vitro studies showed that CA could inhibit tumor growth in seven human cancer cells lines in a concentration-dependent manner,and the IC_ 50 values ranged from 12.3 to 37.1μg·ml~ -1 .In tumor bearing mice,CA and CBP,administered (ip) once daily for 21days,clearly prevented tumor growth in nude mice.The inhibitory rate of CA at 50,100 mg·kg~ -1 and CBP 5 mg·kg~ -1 group was 15.15 %,41.67 %,60.61 % and 65.15% respectively.The number of cells in the S phase of CA treatment increased.Compared with Saline,the apoptosis rate of CA in the 50,100mg·kg~ -1 group increased significantly(P0.05 and P0.01).The human gastric carcinoma xenografts in nude mice treated by CA showed significant morphological changes in different apoptotic phases.Conclusions CA has obvious antitumor effects in vivo and the mechanism relates to proliferation inhibited and induction of apoptosis in cancer cells.