Determination of Endosulfan Residues in Fish,Shrimp and Shellfish by GC

The determination method of endosulfan residues in fish,shrimp and shellfish was developed.The test sample were extracted ultrasonically with mixture solution of acetonitrile and ethyl acetate,condensed in refrigerator at -18 °c to degrease,purified through a complex column loaded with dry sodium sulfate,alumina and florisil successively,determined by gas chromatography and external standard method.The linear range of both α-endosulfan and β-endosulfan were from 0.001 to 0.200 mg/L,the correlation coefficients were respectively for this experiment:α-endosulfan was 0.999 7,β-endosulfan was 0.999 6,validating experiment:α-endosulfan was 0.999 7,β-endosulfan was 0.996 8,and the detection limit of both α-endosulfan and β-endosulfan were 0.001 mg/kg.in the validating tests,the two isomers were added equally into edible tissues of the four kind species at three levels of 0.001,0.010 and 0.050 mg/kg,the recoveries ranged from 77.5 to 108%,rsD ranged from 3.03 to 14.7%.