Effect of Mild Hypothermia on the Content of Malondialdehyde (MDA) and Activities of Superoxide Dismutase (SOD) in Brain Tissue after Complete Cerebral Ischemia Reperfusion in Dogs

Aim: To observe the effect of mild hypothermia on the content of malondialdehyde (MDA) and activities of superoxide dismutase (SOD) in canine brain tissue after cardiac arrest and resuscitation. Metheds: Seventeen dogs were randomly divided into three groups: group A, nonischemic controls (n=5); group B, given the routine cerebral resuscitation treatment after cardiac arrest lasting ten minutes and resuscitation (n=6); and group C, mild hypothermia (34℃) cerebral resuscitation combined with routine treatmetn. The model of cardiac arrest and resuscitation in dogs was made using Tans method. Four hours after cardiac resuscitation, cerebral tissue was sampled for measuring the MDA content and SOD activity. Results: In group B, the content of MDA increased significantly ( P 0.01), the activities of SOD decreased significantly ( P 0.01). In group C, MDA was decreased compared with group B; SOD was substantially unchanged compared with group A ( P 0.05) and was higher than that in group B. Conclusion: Mild hypothermia of global brain after complete cerebral ischemia can significantly inhibit accumulation of lipid peroxidation products and produce antioxidation of brain tissue oneself.