Effect of Injection of Ginkgo Biloba Extract and Dipyridamole on p38MAPK Activation in Rats with Renal Ischemia Reperfusion

Objective:To explore the mechanism of protective effects of Ginkgo biloba extract and Dipyridamole injection(XGD) on renal ischemia-reperfusion injury(RIRI).Methods:The model of renal ischemia-reperfusion injury in male rats was made by removing the right renal,and clamping of left renal artery for 1 hour and 1 hour of reperfusion.The rats with pretreatment were fed with XGD respectively at the dosage of 0.9,1.8,3.6mL/kg prior to operation for a week,The content of malondialdehyde(MDA),the activity of superoxide dismutase(SOD) in renal tissue and the levels of serum creatinine(Scr),blood urea nitrogen(BUN) were measured.The morphologic changes of renal tissue were observed by electronic microscope.western bloting was used to detect the protein expression and activation.Results:After ischemia-reperfusion,the activity of SOD in renal tissue was decreased,however,the content of MDA and p-p38MAPK protein in renal tissue and the levels of BUN,Cr in plasma were increased.Pathological changes induced by ischemia-reperfusion in renal tissues were observed clearly.The pretreatment of rats with XGD significantly prevented reduction of SOD activity and increasing of MDA content and p-p38MAPK protein in renal tissue,decreased elevation of concentration of BUN and Cr in plasma.Pathological changes of proximal tubullar cells in rat kidneys induced by ischemia-reperfusion were also prevented by the pretreatment with XGD.Different XGD dosage groups showed no significant differences.Conclusion:XGD can protect rats from renal injuries caused by ischemia-reperfusion.The mechanism of protective effects of XGD may be related to preserving the activity of SOD and alleviating lipid peroxidation to reduce the expression of p-p38MAPK protein to improve the ischemia-reperfusion injury in rat kidney function and to reduce the damage.