Effect of Different Transplantation Methods of Bone Marrow Mesenchymal Stem Cells on Rats with Liver Cirrhosis

Objective To investigate the effects of transplantation of rat bone marrow mesenchymal stem cells(rBMSCs) through different injection ways on the development of fibrogenesis in rats,and observe the distribution of transplanted BMSCs in the injured liver.Methods BMSCs were isolated and purified by differential adhention methods.The third passage of cells were identified and labeled by green fluorescent protein(GFP) gene transfection.Experimental animals were randomly divided into control and hepatocirrhosis groups.The hepatocirrhosis groups received CCl4 by gavage twice a week for 8 weeks.The GFP labeled-BMSCs were injected into the rats with liver fibrosis by 4 common transplantation ways.All rats were sacrificed 4 weeks after injection and their hepatic function were examined.The degree of liver cirrhosis and the distribution of the infused cells were observed using the liver tissue slices.Results These transplanted BMSCs could survive,migrate to the injured liver,and differently distributed according to the injected ways.The levels of plasma albumin(ALB),alaninotransferase(ALT) and aspartate aminotransferase(AST) were all decreased and the liver fibrosis was further developed in the BMSCs transplanted groups.Conclusion BMSCs transplantation has no therapeutic effects on chronic liver fibrosis of rats,but may participate in the cirrhosis development.