Establishment of a Transgenic Mouse Model of Heme Oxygenase-1 Dominant Negative Mutation

Objective To establish a HO-1 dominant negative mutant G143H transgenic mouse model.Methods The purified HO-1G143H fragment,digested from pCAGGHO-1G143H plasmid with SalI and DraI,was microinjected into FVB superovulated pronuclear zygotes.The injected zygotes were transplanted into the oviducts of pseudopregnant mice.The genotype of transgenic mice was identified by PCR and Southern blot.The HO-1 expression in the tissues of the transgenic mice was detected by RT-PCR,immunohistochemistry and Western blot.Results Three positive transgenic male mice were detected from 17 viable offsprings.RT-PCR,immunohistochemistry and Western blot results demonstrated that the levels of HO-1 mRNA and protein were increased in the positive mice.Conclusion A HO-1 dominant negative mutant HO-1G143H transgenic mouse model has been established successfully.The transgenic mice may serve as a suitable model for the research of HO-1action mechanisms in vivo.