The effection of matrix metalloproteinase and its inhibitor on the expression of MMP-2 and TIMP-1 in paraquat poisoning pulmonary fibrosis rats

Objective To observe the effects of salidroside( SDS) and paraquat( PQ) on the expression of matrix metalloproteinase( MMP- 2) and tissue inhibitor of metalloproteinase- 1( TIMP- 1) in paraquat poisoning pulmonary fibrosis rats.Methods SD rats were randomly divided into three groups,normal control group( NS),paraquat( PQ) model group and salidroside( SDS) intervention group. The pulmonary fibrosis model induced by PQ poisoning was given a gavage of PQ solution,rats in SDS intervention group intraperitoneal injection SDS solution,and normal control group( NS) were given normal saline solution with the same volumeand. The normal control group directly executed rats after anesthesia,PQ model group and SDS intervention group at 1 d,3 d,7 d,14 d,21 d,28 d the rats were sacrificed,and the lungs were taken away for HE staining detecting lung tissue pathological changes; Immunohistochemical method was used to detect MMP- 2 and TIMP- 1 protein expression; Real- Time PCR to detect the expression of MMP- 2 and TIMP- 1 mRNA. Results Compared with NS group,PQ model groups the mRNA and protein expression of MMP- 2 was obviously increased( P 0. 01); RNA and protein expression of TIMP- 1was obviously increased( P 0. 01). Compared with PQ model group,SDS intervention group at each time point lung tissue MMP- 2 and TIMP- 1 protein expression significantly reduced,there is significant difference( P 0. 05). Conclusion SDS can decrease the expression of MMP- 2 and TIMP- 1 mRNA and protein,inhibition of fibrosis in the lung tissue of rats PQ poisoning.