ScholarLens Back to search
2010•PubMedRequires access

[Purification of oleuropein by sephadex LH-20 column].

Jian-Zhang Dang, Xiao-Zhong Nie, Liqin Liang

Open publisher page 1 citations

Abstract

OBJECTIVE: To purify the oleuropein crude extracts by sephadex LH-20 column chromatograph. METHODS: This experiment used fast protein chromatography system (AKTA FPLC) produced by Amersham, Sweden. The chromatography column (20 mm x 300 mm) was matched with protein purification instrument. Sephadex LH-20 was used in the Fast Protein Liquid Chromatography columns. The mobile phase was 50% ethanol with a flow velocity of 1.0 mL per minute and the detection wavelength was 254 nm. The content of oleuropein was determined by HPLC. RESULTS: The purity of oleuropein was 82.9% after passing the column twice when the sample volume was 2 mL. CONCLUSION: Sephadex LH-20 can be re-used and the regeneration is convenient, it also provides a reference for the production of oleuropein.

About this research paper

What this paper is about

OBJECTIVE: To purify the oleuropein crude extracts by sephadex LH-20 column chromatograph. METHODS: This experiment used fast protein chromatography system (AKTA FPLC) produced by Amersham, Sweden. The chromatography column (20 mm x 300 mm) was matched with protein purification instrument. Sephadex LH-20 was used in the Fast Protein Liquid Chromatography columns. The mobile phase was 50% ethanol with a flow velocity of 1.0 mL per minute and the detection wavelength was 254 nm. The content of oleuropein was determined by HPLC. RESULTS: The purity of oleuropein was 82.9% after passing the column twice when the sample volume was 2 mL. CONCLUSION: Sephadex LH-20 can be re-used and the regeneration is convenient, it also provides a reference for the production of oleuropein.

Why it matters

OpenAlex reports 1 citations for this work. Citation counts describe recorded attention and do not establish research quality.

Key contribution

A contribution statement is not available in the OpenAlex record.

Method / approach

Method details are not available in the OpenAlex metadata.

Main findings

Findings are not separately available in the OpenAlex metadata.

Limitations

Limitations are not available in the OpenAlex metadata.

Applications

Application details are not available in the OpenAlex metadata.

Available abstract

OBJECTIVE: To purify the oleuropein crude extracts by sephadex LH-20 column chromatograph. METHODS: This experiment used fast protein chromatography system (AKTA FPLC) produced by Amersham, Sweden. The chromatography column (20 mm x 300 mm) was matched with protein purification instrument. Sephadex LH-20 was used in the Fast Protein Liquid Chromatography columns. The mobile phase was 50% ethanol with a flow velocity of 1.0 mL per minute and the detection wavelength was 254 nm. The content of oleuropein was determined by HPLC. RESULTS: The purity of oleuropein was 82.9% after passing the column twice when the sample volume was 2 mL. CONCLUSION: Sephadex LH-20 can be re-used and the regeneration is convenient, it also provides a reference for the production of oleuropein.

Key concepts: Oleuropein, Chromatography, Chemistry, Sephadex, Chromatography column, High-performance liquid chromatography, Column chromatography, Fast protein liquid chromatography

Related papers

Related paper 1Related paper 2Related paper 3
Back to paper searchBrowse research topicsOriginal source
[Purification of oleuropein by sephadex LH-20 column]. — Research Paper | ScholarLens