Cloning of Tumor Sensitive Related Genes:Construction of a cDNA Library of Suppression Subtractive Hybtridization

Abstruct:Objective To study the new cross muliresist mechanism for HePC Methods The human tumor epitheliacress resistance cell strain KBr that induced by HePC from KB was established and suppression subtractive hybtridization(SSH) was used to screen out differently expressed genes in KB cells in KB cell After enzyme restriction shows that all plasmids in the clones contain 400-800 bp inserts KBr Subtrctive contain library with high subtractive efficency was set up successfully Results The amplified contains 127 positive clones About 78 cDNA clone were obtained form this library by sequencing 27% genes were not find homologus fragment or lower than homologous fragment,may be new genes 14% genes have chromosome locations We think that exclusively chemistry sensitive tumor cDNA fragment KB cell 19% genes MGC and CGAP appear to human EST library May be tumor cell have characterize by genes We find more than 40% genes are known multiresistance genes Conclusions The propose is to explore the of tumor multiresist genes and find new way to reverse multiresistance