Construction of shRNA expression vector system targeting hTERT gene plasmid

Objective To construct the expressing vector of small hairpin RNA(shRNA) targeting human telomerase reverse transcriptase(hTERT) gene.Methods Two template DNA oligonucleotides for shRNA expression targeted hTERT gene were chemically synthesized.The annealed shRNA templates were processed to produce and identify the recombinant RNAi plasmid sPilencer-hTERT.Results It was demonstrated that shRNA template targeting hTERT gene had been inserted at the expected site and the insertion sequence was perfectly correct.Conclusion RNAi expression vector targeting hTERT gene has been constructed successfully and will be used as a useful method to develop specific hTERT-silencing therapeutics.