Effects of a-momorcharin on human trophoblast and deciduacells in vitro

By observing the morphological changes of the human trophoblast and decidua cells as well as the HCG secretion by the trophoblast cells in vitro, we tried to elucidate the cytological mechanism of the anti- early pregnancy effect of α-momorcharin.The results showed that the treated trophoblast cells all had obvious morphological damage in different degrees after 24 h treatment with different concentrations of a-momorcharin (0.13, 0.50, 2.00 and 8.00μg / ml). The degrees of damage were dependent on the concentration and duration, and α-momorcharin showed an inhibitory effect on HCG secretion. The reduction of HCG secretion could be detected as early as6 h after the treatment and the cells almost lost their ability of secreting HCG after 24 h treatment. The decidua cells were also damaged by a-momorcharin, but they were not as sensitive as trophoblast cells.Our results proved that the a-momorcharin treatment could not only directly kill trophoblast cells, but also damage decidua cells. Therefore the α-momorcharin treatment can terminate early pregnancy.