Changes of Ca~(2+)-ATPase in Photosensitive Genic Male Sterile Rice Nong-ken 58S during Pollen Aborting

TEM and lead nitrate were used to locate Ca2+-ATPase in the anther development of photoperiod-sensitive genic male sterile rice Nongken 58S and normal fertile rice Nongken 58N. The results showed that the quantity and the producing time of Ca2+-ATPase in the anther wall, connective tissue, and pollen of fertile and sterile rice were markedly different. Some of Ca2+-ATPase distributed on the epidermal cells of fertile anther at pollen mother cell stage. And there were some Ca2+-ATPase in different anther wall cells, the more on the Ubisch body during the development of the pollen. Comparatively, the less and the late-produced Ca2+-ATPase distributed in different anther wall cells of the sterile anther at the same stage. There were visible Ca2+-ATPase in the vacuole membrane of epidermal cell, cytoplasm of remained tapetum cell, and the surface of Ubisch bodies at the binucleate pollen stage. Abundant Ca2+-ATPase distributed on the exine, the intine, the membrane of cytoplasm, and the nu-cleus of fertile pollen at the late uninucleate pollen stage, but no Ca2+-ATPase distributed on the sterile pollen during the develop-ment of the anther except binucleate pollen stage. The quantity of Ca2+-ATPase in the sterile pollen was less than that in the fertile pollen at the binucleate pollen stage. There were most Ca2+-ATPase in the fertile connective tissue at pollen mother cell meiosis stage. Comparatively, a little Ca2+-ATPase distributed in the sterile connective tissue until the pre-uninucleate pollen stage. We suppose that the reason of pollen abortion is the less and later-produced Ca2+-ATPase distribution on the sterile pollen wall and the plasma membrane during the sterile anther development. Thus, the Ca2+ pump is in low activity to transport Ca2+ leading to pollen abortion in sterile lines due to abundant Ca2+ in the cytoplasm.