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2006•Jiangsu Medical JournalRequires access

Multiplex fluorescence in situ hybridization in detecting complex chromosomal aberrations of multiple myeloma

Jinlan Pan

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Abstract

Objective To evaluate the technique of multiplex fluorescence in situ hybridization(M-FISH) in the detection of the complex chromosomal aberrations(CCAs) in multiple myeloma(MM).Methods M-FISH was used in five MM patients with CCAs detected by R-banding to refine complex chromosomal rearrangements and the constitute of marker chromosomes.Results Twenty kinds of structural rearrangements were detected by M-FISH including 2 kinds of deletions,18 kinds of translocations which were all imbalanced rearrangements.In addition,chromosomes 14,6 and 1q rearrangements were the most frequently involved aberrations.Conclusion M-FISH could refine CCAs of MM patients,find or correct the missed or misidentified abnormalities analysed by conventional cytogenetics.It provides an ideal method for the research of chromosomal aberrations in MM.

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What this paper is about

Objective To evaluate the technique of multiplex fluorescence in situ hybridization(M-FISH) in the detection of the complex chromosomal aberrations(CCAs) in multiple myeloma(MM).Methods M-FISH was used in five MM patients with CCAs detected by R-banding to refine complex chromosomal rearrangements and the constitute of marker chromosomes.Results Twenty kinds of structural rearrangements were detected by M-FISH including 2 kinds of deletions,18 kinds of translocations which were all imbalanced rearrangements.In addition,chromosomes 14,6 and 1q rearrangements were the most frequently involved aberrations.Conclusion M-FISH could refine CCAs of MM patients,find or correct the missed or misidentified abnormalities analysed by conventional cytogenetics.It provides an ideal method for the research of chromosomal aberrations in MM.

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Available abstract

Objective To evaluate the technique of multiplex fluorescence in situ hybridization(M-FISH) in the detection of the complex chromosomal aberrations(CCAs) in multiple myeloma(MM).Methods M-FISH was used in five MM patients with CCAs detected by R-banding to refine complex chromosomal rearrangements and the constitute of marker chromosomes.Results Twenty kinds of structural rearrangements were detected by M-FISH including 2 kinds of deletions,18 kinds of translocations which were all imbalanced rearrangements.In addition,chromosomes 14,6 and 1q rearrangements were the most frequently involved aberrations.Conclusion M-FISH could refine CCAs of MM patients,find or correct the missed or misidentified abnormalities analysed by conventional cytogenetics.It provides an ideal method for the research of chromosomal aberrations in MM.

Key concepts: Fluorescence in situ hybridization, Chromosomal translocation, Fish <Actinopterygii>, Multiplex, Biology, Cytogenetics, Molecular biology, Karyotype

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