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2006•Unpublished venueRequires access

Role of NF-kappa B in regulating expression of ICAM-1

Zeng Bangxiong

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Abstract

AIM: To study the role of NF-kappa B in regulating the expression of adhesion molecule ICAM-1.METHODS: HUVECs were isolated from human umbilical veins by(0.25)% trypsin and then cultured in RPMI1640,exposed on LPS(100(ng·ml~(-1))) for different time(1,4,8,and 24 h),while pretreated by NF-kappa B inhibitor pyrrolidine dithiocarbamate(PDTC) at the 4th hour.Nucleus protein and total RNA were extracted.The activity of NFkappa B and the expression of ICAM-1 were measured respectively by the electrophoretic mobility shift assay(EMSA) and reverse transcriptase-PCR(RT-PCR).RESULTS: The activity of NF-kappaB altered in correlate with ICAM-1.Treating HUVECs with PDTC,the expression of ICAM-1mRNA was significantly downregulated with the decline activity of NF-kappa B((2.40)±(0.11) vs(0.76)±(0.09),P(0.01)).CONCLUSION: NF-kappa B plays an important role in regulating the expression of ICAM-1Mrna.

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AIM: To study the role of NF-kappa B in regulating the expression of adhesion molecule ICAM-1.METHODS: HUVECs were isolated from human umbilical veins by(0.25)% trypsin and then cultured in RPMI1640,exposed on LPS(100(ng·ml~(-1))) for different time(1,4,8,and 24 h),while pretreated by NF-kappa B inhibitor pyrrolidine dithiocarbamate(PDTC) at the 4th hour.Nucleus protein and total RNA were extracted.The activity of NFkappa B and the expression of ICAM-1 were measured respectively by the electrophoretic mobility shift assay(EMSA) and reverse transcriptase-PCR(RT-PCR).RESULTS: The activity of NF-kappaB altered in correlate with ICAM-1.Treating HUVECs with PDTC,the expression of ICAM-1mRNA was significantly downregulated with the decline activity of NF-kappa B((2.40)±(0.11) vs(0.76)±(0.09),P(0.01)).CONCLUSION: NF-kappa B plays an important role in regulating the expression of ICAM-1Mrna.

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Available abstract

AIM: To study the role of NF-kappa B in regulating the expression of adhesion molecule ICAM-1.METHODS: HUVECs were isolated from human umbilical veins by(0.25)% trypsin and then cultured in RPMI1640,exposed on LPS(100(ng·ml~(-1))) for different time(1,4,8,and 24 h),while pretreated by NF-kappa B inhibitor pyrrolidine dithiocarbamate(PDTC) at the 4th hour.Nucleus protein and total RNA were extracted.The activity of NFkappa B and the expression of ICAM-1 were measured respectively by the electrophoretic mobility shift assay(EMSA) and reverse transcriptase-PCR(RT-PCR).RESULTS: The activity of NF-kappaB altered in correlate with ICAM-1.Treating HUVECs with PDTC,the expression of ICAM-1mRNA was significantly downregulated with the decline activity of NF-kappa B((2.40)±(0.11) vs(0.76)±(0.09),P(0.01)).CONCLUSION: NF-kappa B plays an important role in regulating the expression of ICAM-1Mrna.

Key concepts: Pyrrolidine dithiocarbamate, ICAM-1, Electrophoretic mobility shift assay, NF-κB, NFKB1, Chemistry, Kappa, Molecular biology

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