CHANGES OF FIBROBLASTS AFTER ISCHEMIA/REPERFUSION IN EXTRAHEPATIC BILE DUCT IN RATS

Objective To investigate the temporal changes of fibroblast apoptosis and gene expression during extrahepatic bile duct ischemia/reperfusion. Methods At 1,3,24,48 and 72?h after 30?min's extrahepatic bile duct ischemia in 50 rats,apoptotic index of fibroblasts was calculated by TUNEL staining,the expression of bcl-2 mRNA was evaluated by in situ hybridization and Fas and proliferating cell nuclear antigen(PCNA) expression were evaluated by immunohistochemistry. Results At the first hour after ischemia/reperfusion,no significant changes were observed in the expression of Fas,PCNA and Bcl-2 mRNA between the ischemic and the control groups.The increase of apoptotic index of fibroblast was first seen at 3h after reperfusion,and up to peak at 24?h and declined gradually thereafter.The expression of Fas concurred with the apoptotic index.The expression of PCNA appeared at 3?h after reperfusion and remained high from 24?h to 72?h.The expression of Bcl-2 mRNA peaked at 24?h after reperfusion.Conclusion The extrahepatic bile duct ischemia/reperfusion resulted in both apoptosis and proliferation of fibroblast,and apoptosis only appeared at early phase but proliferation maintained to the end of the experiment.