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Effect of Pretreatment with Low Temperature on Embryogenesis of Immature Cotyledon in Soybean

Ping Wang

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Abstract

The effects of pretreatment with 4℃ low temperature(0,2,3,4,5 and 6 days respectively) on somatic embryogenesis were conducted before immature cotyledons were cultured with 3 varieties in soybean ( Glycine max L.). The results showed that the pretreatment with 4℃ low temperature (3 or 4 days )could stimulate somatic embryogenesis and raise frequency of embryogenesis. Three varieties were similar in reaction to pretreatment with low temperature.

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What this paper is about

The effects of pretreatment with 4℃ low temperature(0,2,3,4,5 and 6 days respectively) on somatic embryogenesis were conducted before immature cotyledons were cultured with 3 varieties in soybean ( Glycine max L.). The results showed that the pretreatment with 4℃ low temperature (3 or 4 days )could stimulate somatic embryogenesis and raise frequency of embryogenesis. Three varieties were similar in reaction to pretreatment with low temperature.

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Available abstract

The effects of pretreatment with 4℃ low temperature(0,2,3,4,5 and 6 days respectively) on somatic embryogenesis were conducted before immature cotyledons were cultured with 3 varieties in soybean ( Glycine max L.). The results showed that the pretreatment with 4℃ low temperature (3 or 4 days )could stimulate somatic embryogenesis and raise frequency of embryogenesis. Three varieties were similar in reaction to pretreatment with low temperature.

Key concepts: Cotyledon, Somatic embryogenesis, Embryogenesis, Glycine, Biology, Embryo, Chemistry, Horticulture

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