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2005•Chinese journal of integrated traditional and Western medicineRequires access

Effect of the Hepatitis B Virus X Gene Transfection on Apoptosis of HepG2 and Its Mechanism

Hong Chen

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Abstract

Objective: To investigate HBxs effect on apoptosis of HepG2 and its effect on expression of apoptosis factors. Methods: The HBV X gene eukaryon expression vector pcDNA 3 X was transiently transfected into HepG2 cell by lipid media transfection. Untrasfected HepG2 and HepG2 transfected with pcDNA 3 were used as control. The expression of HBx in HepG2 was identified by RT PCR. MTT and TUNEL were employed to detect proliferation and apoptosis of three groups. Semi quantified RT PCR was used to evaluate the expression of Bax, Bcl xL, c myc in three groups, and β actin was used as report gene.Results: Proliferative capacity in HepG2/pcDNA 3 X was obviously decreased, accompanied with high rate of apoptosis. Compared with HepG2 and HepG2/pcDNA 3 cell,expression of Bax, Bcl xL, c myc in HepG2/pcDNA 3 X were up regulated.Conclusion:HBx can up regulate either Bax, c myc and Bcl xL. This effection results in cell apoptosis and impairs proliferative capacity of cell.

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Objective: To investigate HBxs effect on apoptosis of HepG2 and its effect on expression of apoptosis factors. Methods: The HBV X gene eukaryon expression vector pcDNA 3 X was transiently transfected into HepG2 cell by lipid media transfection. Untrasfected HepG2 and HepG2 transfected with pcDNA 3 were used as control. The expression of HBx in HepG2 was identified by RT PCR. MTT and TUNEL were employed to detect proliferation and apoptosis of three groups. Semi quantified RT PCR was used to evaluate the expression of Bax, Bcl xL, c myc in three groups, and β actin was used as report gene.Results: Proliferative capacity in HepG2/pcDNA 3 X was obviously decreased, accompanied with high rate of apoptosis. Compared with HepG2 and HepG2/pcDNA 3 cell,expression of Bax, Bcl xL, c myc in HepG2/pcDNA 3 X were up regulated.Conclusion:HBx can up regulate either Bax, c myc and Bcl xL. This effection results in cell apoptosis and impairs proliferative capacity of cell.

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Available abstract

Objective: To investigate HBxs effect on apoptosis of HepG2 and its effect on expression of apoptosis factors. Methods: The HBV X gene eukaryon expression vector pcDNA 3 X was transiently transfected into HepG2 cell by lipid media transfection. Untrasfected HepG2 and HepG2 transfected with pcDNA 3 were used as control. The expression of HBx in HepG2 was identified by RT PCR. MTT and TUNEL were employed to detect proliferation and apoptosis of three groups. Semi quantified RT PCR was used to evaluate the expression of Bax, Bcl xL, c myc in three groups, and β actin was used as report gene.Results: Proliferative capacity in HepG2/pcDNA 3 X was obviously decreased, accompanied with high rate of apoptosis. Compared with HepG2 and HepG2/pcDNA 3 cell,expression of Bax, Bcl xL, c myc in HepG2/pcDNA 3 X were up regulated.Conclusion:HBx can up regulate either Bax, c myc and Bcl xL. This effection results in cell apoptosis and impairs proliferative capacity of cell.

Key concepts: HBx, Transfection, Apoptosis, TUNEL assay, Molecular biology, Hepatitis B virus, Cell growth, Biology

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