Genetic analysis and preliminary gene mapping of a novel seeding yellowleaf mutant in rice

A novel seeding yellow-leaf mutant, syl11(seedling yellow leaf 11),was found in rice mutant pool from japonica variety,Nipponbare,treated by 60Coγ-radiation.In comparison with the wild-type parent (Nipponbare),the mutant displayed the phenotype of yellow on the 2nd and 3rd leaf and began to turn green from its top before they completely expanded besides the significantly decreased contents of photosynthetic pigments,but all leaves become normal green after the 4-leaf-old stage.The genetic analysis indicated that the mutant trait was controlled by a single recessive nuclear gene.In addition,with the help of SSR,new developed InDel markers and F2 segregating population derived from the cross between the syl11 mutant and Peiai64S,the syl11 was mapped between RM26652(on the long arm)and ID11974(closing to the centromere)on chromosome 11,with a genetic distance of 0.5cM and 0.7cM,respectively.