Effects of ceruloplasmin on brain edema after intracerebral hemorrhage in rats

Objective To examine whether exogenous ceruloplasmin reduces brain edema induced after intracerebral hemorrhage (ICH). Methods Male Sprague-Dawley rats received an infusion of 100 μl autologous blood or 100 μl autologous blood plus 5 μM ceruloplasmin into the right basal ganglia. The brain water and ion content in rat brain were measured 3 days,7 days after ICH. Behavioral tests (corner turn test, forelimb placing test) were used to examine neurological deficits. Results After intracerebral hemorrhage and 7 days, 3 days with CP, water content, Na+ content of the brain tissue of basal ganglia reduced with statistical significance(t=2.13,2.56,4.57, 3.51, P0.05). After hemorrhage and 3 days with CP, neurological recovery was obviously improved. Especially in 3 days, 21 days and 30 days, the results of corner test and forelimb placing test had significant differences between the hemorrhage group and CP group(t=1.82,1.79,2.03,2.16,2.03,3.03, P0.05). Conclusions Ceruloplasmin may be an important point in brain edema formation after ICH.