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2009•Chinese Journal of Health Laboratory TechnologyRequires access

Determination of protein in milk by spectrophotometry after decomposition under pressurized condition

Li Tao

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Abstract

Objective:To establish a mathod for determination of protein in milk by spectrophotometry.Methods:After the milk was decomposed with H2SO4(1+1) under pressurized condition(115℃~116℃,0.17~0.18Mpa,1 h),the amino acids reacted with ninhydrin to form colored products and determined by spectrophotometry.Results:The linear range of the method was 0.10~0.60 mg/ml(r=0.9991).The detection limit was 0.02 mg/ml with the recoveries of 91%~101.7% and its RSD was 0.84%~2.23%.

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Objective:To establish a mathod for determination of protein in milk by spectrophotometry.Methods:After the milk was decomposed with H2SO4(1+1) under pressurized condition(115℃~116℃,0.17~0.18Mpa,1 h),the amino acids reacted with ninhydrin to form colored products and determined by spectrophotometry.Results:The linear range of the method was 0.10~0.60 mg/ml(r=0.9991).The detection limit was 0.02 mg/ml with the recoveries of 91%~101.7% and its RSD was 0.84%~2.23%.

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Available abstract

Objective:To establish a mathod for determination of protein in milk by spectrophotometry.Methods:After the milk was decomposed with H2SO4(1+1) under pressurized condition(115℃~116℃,0.17~0.18Mpa,1 h),the amino acids reacted with ninhydrin to form colored products and determined by spectrophotometry.Results:The linear range of the method was 0.10~0.60 mg/ml(r=0.9991).The detection limit was 0.02 mg/ml with the recoveries of 91%~101.7% and its RSD was 0.84%~2.23%.

Key concepts: Spectrophotometry, Ninhydrin, Chromatography, Chemistry, Detection limit, Infrared Spectrophotometry, Linear range, Decomposition

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