Determination of Anthocyanin in Merlot Grape and in Pinot Noir Grape by HPLC
Yaxin Sang
Abstract
Yaxin Sang
Abstract
Anthocyanin in Merlot grape and in Pinot Noir grape was extracted by methanol and ultrasonic and then it was detected by HPLC.9 kinds of anthocyanins were detected in Merlot grape mainly including Malvidin 3-O-Glu,Malvidin 3-O-(6-O-Acetyl)-Glu and Malvidin 3-O-(6-O-P-Coumaryl)-Glu which accounted for 76.69 % of the total anthocyanins and were the main coloration compositions of Merlot grape.Only 5 kinds of anthocyanins were detected in Pinot Noir grape mainly including Malvidin 3-O-Glu and Peonidin 3-O-Glu(the main coloration compositions) which accounted for 94.18 % of the total anthocyanins.
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Anthocyanin in Merlot grape and in Pinot Noir grape was extracted by methanol and ultrasonic and then it was detected by HPLC.9 kinds of anthocyanins were detected in Merlot grape mainly including Malvidin 3-O-Glu,Malvidin 3-O-(6-O-Acetyl)-Glu and Malvidin 3-O-(6-O-P-Coumaryl)-Glu which accounted for 76.69 % of the total anthocyanins and were the main coloration compositions of Merlot grape.Only 5 kinds of anthocyanins were detected in Pinot Noir grape mainly including Malvidin 3-O-Glu and Peonidin 3-O-Glu(the main coloration compositions) which accounted for 94.18 % of the total anthocyanins.
Key concepts: Anthocyanin, Malvidin, Peonidin, Chemistry, Grape wine, Cyanidin, Food science, High-performance liquid chromatography