Primary Culture Method of Endothelial Cells Derived from Human Umbilical Vein

To establish a method of culturing primary cells derived from human umbilical vein,endothelial cells of human umbilical vein were digested with collagenase Ⅱ,cultured in DMEM medium with 10% FBS,with or withnot EGF(epidermal growth factor),followed by immunocytochemical technique to verify HUVEC.The HUVEC grow the fastest in 3～4 days,in a pattern of contact inhibition,showing a cobblestone or pitching stone-like appearance.It was proved that high purity EC can be harvested with digestion of collagenase Ⅱperfusion with high survival rate.This method provides experimental model for vascular EC research.