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Expression of Horseradish Peroxidase Isozyme C3 Gene in Pichia pastoris

Jianmei Wu

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Abstract

Objective:To clone and express the horseradish peroxidase isozyme C3(HRPC3) gene.Methods: The HRPC3 gene was amplified with PCR from the genomic DNA of horseradish,and it was then linked into pMD-18T vector.After it was confirmed by sequencing analysis,it was correctly inserted into the expression vector pPIC9K of Pichia pastoris.Then the recombined pPIC9K plasmid with target gene was expressed and analyzed.Results: The HRPC3 gene was expressed successfully by using Pichia pastoris as host strain and the activity of it is 56 IU/L.Conclusion: The HRPC3 with biological activity was expressed directly in Pichia pastoris.

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What this paper is about

Objective:To clone and express the horseradish peroxidase isozyme C3(HRPC3) gene.Methods: The HRPC3 gene was amplified with PCR from the genomic DNA of horseradish,and it was then linked into pMD-18T vector.After it was confirmed by sequencing analysis,it was correctly inserted into the expression vector pPIC9K of Pichia pastoris.Then the recombined pPIC9K plasmid with target gene was expressed and analyzed.Results: The HRPC3 gene was expressed successfully by using Pichia pastoris as host strain and the activity of it is 56 IU/L.Conclusion: The HRPC3 with biological activity was expressed directly in Pichia pastoris.

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Available abstract

Objective:To clone and express the horseradish peroxidase isozyme C3(HRPC3) gene.Methods: The HRPC3 gene was amplified with PCR from the genomic DNA of horseradish,and it was then linked into pMD-18T vector.After it was confirmed by sequencing analysis,it was correctly inserted into the expression vector pPIC9K of Pichia pastoris.Then the recombined pPIC9K plasmid with target gene was expressed and analyzed.Results: The HRPC3 gene was expressed successfully by using Pichia pastoris as host strain and the activity of it is 56 IU/L.Conclusion: The HRPC3 with biological activity was expressed directly in Pichia pastoris.

Key concepts: Pichia pastoris, Horseradish peroxidase, Isozyme, Gene, Molecular biology, Plasmid, Recombinant DNA, genomic DNA

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