2008•Food Science and Technology InternationalRequires access
The protoplasts resistance-chemicals mutational labelling selection model of the streptomyces 702
TU Guo-quan
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Abstract
In order to increase the yield of antifungi substance,UV induced-mutation and the protoplasts resistance-gentamycin mutational labelling selection model was used to screen high-yield mutants of streptomyces 702.The results showed that the gentamycin-resistant mutational frequency of the protoplasts of streptomyces 702 treated by UV with 40 seconds was up to 20.18% and the lethal rate was 67.15%.High antifungi substance-producing mutant 42-23-111 was isolated in the screening experiments,and its yield of antifungi substance reached 949 μg/mL,which was 23.2% higher than that produced by its parent strain.