ITS Sequence analysis on four fungi of Hymenochaetaceae

Objective To analyze the rDNA ITS sequences of Inonotus obliquus, Phellinus rimosus, Porodaedalea chrysoloma, and Inonotus baumii, and study the utility in phylogenesis and identification of these strains between China and foreign countries. Methods The ITS gene fragments of the four fungi were PCR amplified and sequenced. The rDNA ITS regions were analyzed by means of the software of Clustal X and Mega 3.1. Results The entire sequences of rDNA ITS1, ITS2, and 5.8S rDNA were obtained. The sequences of ITS1 in these four fungi ranged from 244 to 324 bp in length and those of ITS2 from 229 to 274 bp. The phylogenetic relationships of four sequenced ITS sequences together with those of three selected fungi and Bondarzewia montana (DQ200923) downloaded from GenBank were constructed, B. montana was designated as outgroup. The analysis result was consistent with those from morphology. Conclusion The ITS sequence can be used to identify the molecule of these four medicinal fungi.